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SRX10993599: RNA-Seq of Lolium perenne: Inflorescence primordium, Plant 83, Low transmission
1 ILLUMINA (Illumina HiSeq 2500) run: 86.8M spots, 21.7G bases, 7Gb downloads

Design: Samples (~50 mg) were powdered in liquid nitrogen using a micropestle in a 1.5 mL tube. The RNA extraction method used was a combination of an organic extraction from each sample using Trizol (Thermo Fisher Scientific, Waltham, USA) followed by further purification of 200 L of the Trizol/chloroform aqueous phase with an RNeasy MinElute Cleanup Kit (Qiagen, Valencia, USA). A TruSeq stranded mRNA sample preparation kit (Illumina, San Diego, CA, USA) was used to generate sequencing libraries from the total RNA extractions, and the indexed libraries were pooled and sequenced on two lanes of an Illumina HiSeq 2500 instrument at New Zealand Genomics Limited, Otago, New Zealand.
Submitted by: AgResearch NZ Limited
Study: Plant disease resistance mechanisms are associated with the efficiency of Epichloe endophyte seed transmission in Lolium perenne
PRJNA732816 • SRP321377 • All experiments • All runs
show Abstracthide Abstract
Endophyte transmission in perennial ryegrass is important for plant survival and commercial reasons but the underlying biological processes are poorly understood. We aim to understand the underlying biological processes of endophyte transmission in high- and low-transmitting perennial ryegrass by using transcriptomics and other methods.
Sample: Plant 83 inflorescence primordium
SAMN19341590 • SRS9067982 • All experiments • All runs
Organism: Lolium perenne
Library:
Name: IP_83L
Instrument: Illumina HiSeq 2500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: RANDOM
Layout: PAIRED
Runs: 1 run, 86.8M spots, 21.7G bases, 7Gb
Run# of Spots# of BasesSizePublished
SRR1465508186,788,28421.7G7Gb2022-03-03

ID:
14621736

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